weak D type 4.0

Structure

RHD(T201R,F223V)[819G>A]

Key changes from standard allele

602C>G (T201R)
667T>G (F223V)
819G>A

ISBT allele designation

RHD*09.03.01
RHD*DAR3

Nucleotide changes relative to "standard RHD"

c.[602 C>G;667 T>G;819 G>A]

Amino acid changes relative to standard protein

T201R, F223V, A273A

Haplotype (typical)

cDe

Allele cluster

weak D type 4 cluster

Descriptions

First full publication: 1999 Wagner FF et. al. Blood. 1999 Jan 1;93(1):385-93.

Other relevant descriptions:
alterations in non-coding region: 336-56 delATGA, 802-16C>T, 1227+55A>G KR260900 (submitted 2015-04-20, released 2016-07-04)
KR260901 (submitted 2015-04-20, released 2017-03-07)

ISBT group

partial RHD

Phenotype characterization and grouping

weak D type; weakened D expression; Partial D

Serology

Rhesus Index 0.36 Wagner FF et. al. Blood. 2000 Apr 15;95(8):2699-708.
Kormoczi GF et. al. Transfusion. 2005 Oct;45(10):1574-80.
allo-anti-D observed Rhesus immunization registry

Observations

Brazil (general): 81 of 369 samples with atypical D expression Arnoni CP et. al. Transfusion. 2014 Apr;54(4):962-9. doi: 10.1111/trf.12557. Epub 2014 Feb 28.
South-Western Germany: Wagner FF et. al. Blood. 1999 Jan 1;93(1):385-93. Frequency 1:14.925
Tyrol: 4 of 130 weak D samples Muller TH et. al. Transfusion. 2001 Jan;41(1):45-52.
African American: SWiTCH trial Chou ST et. al. Blood Adv. 2017 Aug 3;1(18):1414-1422. doi: 10.1182/bloodadvances.2017007898. Frequency 1:0,028
African ancestry (general): possibly frequent among Africans Hemker MB et. al. Blood. 1999 Dec 15;94(12):4337-42.
Northern Germany: 1 of 159 weak D samples Muller TH et. al. Transfusion. 2001 Jan;41(1):45-52.
Tunisia (general): 1 of 448 seemingly D negative samples Moussa H et. al. Transfus Med. 2012 Mar 16. doi: 10.1111/j.1365-3148.2012.01142.x.

Additional comments

This allele was initially described as weak D type 4 and has a much lower alloimmunization potential than the "true" DAR-alleles carrying I342T. Although the original DAR publication investigated a sample with a considerably different phenotype and reported an incorrect sequence, the ISBT chose to use the DAR naming for the whole RHD(T201R, F223V) related branch.

Last update: 2018-02-19 (yyyy-MM-dd)