weak D type 2



Key changes from standard allele

1154G>C (G385A)

ISBT allele designation

RHD*weak D type 2

Nucleotide changes relative to "standard RHD"

c.1154 G>C

Amino acid changes relative to standard protein

Gly to Ala at codon 385


Haplotype (typical)


Allele cluster

Eurasian D cluster


First full publication: 1999 Wagner FF et. al. Blood. 1999 Jan 1;93(1):385-93.

ISBT group

weak D

Phenotype characterization and grouping

weak D type; weakened D expression


Rhesus Index 0.68 (59 antibodies, 1 sample) Wagner FF et. al. Blood. 2000 Apr 15;95(8):2699-708.
Rhesus Index 0.61 (1 sample, 22 to 50 antibodies, 12 laboratories) Flegel WA et. al. Transfus Clin Biol. 2002 Jan;9(1):33-42.
Kormoczi GF et. al. Transfusion. 2005 Oct;45(10):1574-80.
May cause anti-D immunization of recipients Flegel WA et. al. Transfusion. 2000 Apr;40(4):428-34.
Variable reactivity 3+ to 0+ (mostly 2+) in routine typing. 6 of 91 samples had anti-D, in all cases auto-anti-D. Pham BN et. al. Transfusion. 2011 Dec;51(12):2679-85.


Australia (general): Frequent among Australian weak D samples Cowley NM et. al. Vox Sang. 2000;79(4):251-2.
Brazil (general): 25 of 369 samples with atypical D expression Arnoni CP et. al. Transfusion. 2014 Apr;54(4):962-9. doi: 10.1111/trf.12557. Epub 2014 Feb 28.
France (general): Second most frequent allele found among weak and partial D samples in Franc Ansart-Pirenne H et. al. Transfusion. 2004 Sep;44(9):1282-6.
Northern Germany: 17% of weak D samples Muller TH et. al. Transfusion. 2001 Jan;41(1):45-52.
South-Western Germany: Wagner FF et. al. Blood. 1999 Jan 1;93(1):385-93. Frequency 1:1.082
Tyrol: 8% of weak D samples Muller TH et. al. Transfusion. 2001 Jan;41(1):45-52.
Croatia (general): Relatively rare (3.6% of weak D samples) in Croatia Dogic V et. al. Transfus Med. 2011 Aug;21(4):278-9.
Poland (general): population frequency 1:10400; observed in Ccee (?? - several surprising haplotype assingments in publication) Orzinska A et. al. Transfusion. 2013 Nov;53(11 Suppl 2):2945-53. doi: 10.1111
Canada (general): 1 of 26 samples with weak or variant D St-Louis M & et al. Immunohematology 2011; 27:20-24

Additional comments

Last update: 2018-02-19 (yyyy-MM-dd)